Journal: Scientific Reports

Article Title: Cathepsins L and B target HIF1α for oxygen-independent proteolytic cleavage

doi: 10.1038/s41598-024-65537-9

Figure Lengend Snippet: HIF1αODD cleavage is reduced by cathepsins L or B inhibitors. ( a – c ). Rabbit reticulocyte lysate (RRL) was pre-incubated for 5 min at either 37 °C or 60 °C, with DMSO or inhibitor, as indicated. Recombinant HIF1αODD-GFP was added and incubated for 60 min at 37 °C. Reactions were resolved by SDS-PAGE and immunoblotted for GFP. E-64 was used at 10 μM; Q-VD-OPh, Z-VAD-FMK, calpastatin peptide, MDL-28170 calpeptin, CA-074 (CTSB inhibitor), LY3000328 (CTSS inhibitor), SB 412515 (CTSL inhibitor), and L-006,235 (CTSK inhibitor) were each used at 0.1, 1, and 10 μM. Blots are representative of three independent experiments. Original blots are presented in Supplementary Fig. .

Article Snippet: Z-VAD-FMK (Cat. # ALX-260-020-M001), Q-VD-OPh (Cat. # S7311), acetyl-calpastatin 184–210 (Cat. # 2950), MDL-28170 (Cat. # S7394), calpeptin (Cat. # 0448), LY3000328 (Cat. # 29729), SB 412515 (Cat. # 23249), L-006,235 (Cat. # 28843), recombinant human cathepsin B (Cat.# 28843), and recombinant human cathepsin L (Cat.# 787504) were obtained from Cedarlane Laboratories.

Techniques: Incubation, Recombinant, SDS Page

Journal: AMB Express

Article Title: φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3

doi: 10.1186/s13568-021-01341-2

Figure Lengend Snippet: Strains used to perform ELISA assay

Article Snippet: 3 , Yersinia enterocolitica DSMZ 23,249 , O:8 , DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.

Techniques: Enzyme-linked Immunosorbent Assay